Fractionation of proteins from natural coagulant from common bean on ion-exchanger AmberliteTM IRA 900 Cl and comparison with AmberliteTM IRA 958 Cl
DOI:
https://doi.org/10.25083/rbl/28.5/4101.4112Keywords:
natural coagulant, proteins, water clarification, coagulation activity, organic load, ion-exchangeAbstract
Turbidity reduction in water and wastewater treatment typically involves chemical coagulants like alum, iron salts, and acrylamide. While natural coagulants offer benefits, their main drawback is the organic load in treated water, which can be addressed through purification. In this work, proteins from a crude extract obtained with 0.5 mol L-1 NaCl from Phaseolus vulgaris seeds were first precipitated by ammonium sulfate and then redissolved and purified on the anion exchange resin AmberliteTM IRA 900 Cl in batch mode. Prior to their purification, optimization of adsorption conditions and elution was performed. The purification on AmberliteTM IRA 900 Cl was then compared with purification on AmberliteTM IRA 958 Cl. AmberliteTM IRA 900 Cl showed twice the adsorption capacity of AmberliteTM IRA 958 Cl. The organic load in treated water was over five times lower when the fraction purified on AmberliteTM IRA 900 Cl was used as a coagulant compared to when the crude extract was used.
