Angiotensinogen: in-silico insight to mutations, protein-protein interactions and evolution

Abstract

In this era of rapidly evolving genetic and non-genetic diseases, in-silico analysis of disease related proteins has proven beneficial in identification and characterization of potential therapeutic targets. In present study, angiotensinogen (AGT) was studied for its sequence based ortholog and paralogs, structural characterization, mutational analysis, protein interactions and evolutionary changes. Sequence studies identified 194 orthologs and 36 paralogs of AGT. Functional analysis AGT polymorphisms revealed the association of M235T with increased AGT stability. Protein-protein interaction analysis exposed the involvement of AGT in metabolic diseases. So, this study provided base for the experimentally proven association of M235T polymorphism with metabolic diseases. Moreover, it presents AGT as strong therapeutic target for the control of these diseases.