Adipose Tissue-derived Cardiomyocytes for Enhancing Cardiac Remodeling in a Rat Model of Acute Myocardial Infarction

Authors

  • MOHAMED S. KISHTA Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affiliation ID: 60014618 Author
  • MOHAMED RAGAA MOHAMED Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt Author
  • HADEER A. AGLAN Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affiliation ID: 60014618 Author
  • MOHAMED A.M. ALI Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt Author
  • HANAA H. AHMED Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affiliation ID: 60014618 Author

DOI:

https://doi.org/10.25083/rbl/27.1/3315-3329

Keywords:

Adipose tissue-derived cardiomyocytes, Electro-spun nanofibers, In vitro, In vivo, Myocardial infarction

Abstract

This investigation was directed towards addressing the pivotal role of adipose tissue-derived cardiomyocytes seeded onto nanofiber (NF) in repairing the  deteriorated cardiac tissue in a model of MI. Molecular analysis for MEF2C and  Actn expression was attained to ensure the differentiation of ADMSCs into  cardiomyocytes in vitro. The in vivo study was conducted on forty adult rats  assigned into four groups: (1) control; (2) MI; (3) MI treated with adipose tissue- derived cardiomyocytes; (4) MI treated with adipose tissue-derived cardiomyocytes  seeded onto NF. Treatment of MI-challenged rats with adipose  tissue-derived cardiomyocytes modulates ST height, heart rate, RR, PR, QTc, QRS intervals and P duration as manifested in the ECG. The biochemical parameters  corroborated the ECG outcomes as they displayed significant inhibition in serum  LDH and CK-MB enzymes activity as well as significant suppression in cardiac cTnT  level paralleled with significant elevations in cardiac Cx43 and Actn levels in the  treated groups. Molecular analysis of GATA4 and NKX2.5 gene expression levels  declared significant downregulation in the treated groups. Photomicrographs of  cardiac tissue sections of rats in the treated groups showed great renovation in the  cardiac microarchitecture. Conclusively, this study delivers a futuristic  approach for treatment of MI by applying differentiated cardiomyocytes  systematically. 

Author Biographies

  • MOHAMED S. KISHTA, Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affiliation ID: 60014618

    Stem Cell Lab., Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt,
    Affiliation ID: 60014618

  • HADEER A. AGLAN, Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affiliation ID: 60014618

    Stem Cell Lab., Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt,
    Affiliation ID: 60014618

  • HANAA H. AHMED, Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affiliation ID: 60014618

    Stem Cell Lab., Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt,
    Affiliation ID: 60014618

RBL271-17

Downloads

Published

2024-05-23

Issue

Vol. 27 No. 1 (2022): Romanian Biotechnological Letters

Section

Articles