Mesenchymal stem cells attenuate amiodarone-induced pulmonary fibrosis in rats via blockade of inflammation and TGF-B1/Smad3/ S100A4 signaling

Authors

  • ALYAA S. ABDEL HALIM, Ph.D. Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt Author
  • HANAA H. AHMED, Ph.D. Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affliation ID: 60014618 Author
  • HADEER A. AGLAN, Ph.D. Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affliation ID: 60014618 Author
  • MOHAMED R. MOHAMED, Ph.D. Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt Author

DOI:

https://doi.org/10.25083/rbl/27.2/3368.3378

Keywords:

Amiodarone, conditioned media, inflammatory response, lung fibrosis, mesenchymal stem cells, TGF-B1 signaling

Abstract

Aim: This study aimed to clarify the anti-fibrotic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs), compared to conditioned media (CM), in  amiodarone (AD)-induced lung fibrosis. Methods: A total of 64 Wistar rats were categorized into eight groups: negative  control group, positive control group, 3 AD-challenged-BM-MSCs-treated groups (1, 2 and 4 months) and 3 AD-challenged-CM-treated groups (1, 2 and 4 months). Serum macrophage inflammatory protein 2 (MIP-2) levels were measured. Gene expression levels of TGF-B1, SMAD3 and S100A4, were estimated in the lung tissues. Results: Treatment with BM-MSCs/CM mediated a significant reduction in serum MIP-2 concentrations, while  downregulating AD-induced up-regulation of lung TGF-B1, SMAD3 and S100A4 gene expression levels. BM-MSCs transplantation revealed better effect than CM in mitigating lung fibrosis. Conclusion: BM-MSCs advance anti-fibrotic effect on lung fibrosis by targeting inflammatory response and TGF-B1 signaling.

Author Biographies

  • HANAA H. AHMED, Ph.D., Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affliation ID: 60014618

    Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt,
    Affliation ID: 60014618

  • HADEER A. AGLAN, Ph.D., Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Giza, Egypt, Affliation ID: 60014618

    Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt,
    Affliation ID: 60014618

RBL272-5

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Published

2024-05-23

Issue

Vol. 27 No. 2 (2022): Romanian Biotechnological Letters

Section

Articles