Characterization of a lipolytic strain of Pseudomonas stutzeri SN-3 and production of triacylglycerol hydrolase with concomitant biodegradation of palm oil

Abstract

triglycerides. Lipases are the ubiquitous enzymes with their applications ranging from food industry to cosmetics, pharmaceuticals and bioremediation purposes. The present research involves 16S rDNA sequencing of a lipase producing strain isolated indigenously. The strain was identified as a novel Pseudomonas stutzeri  SN-3, the gene sequence of which was deposited in GenBank with accession  number MH639065. The research design also includes the exploration of  alternative fermentation conditions for maximum production of triacylglycerol hydrolase from the novel strain. Different physical and chemical parameters were  studied which includes temperature, pH, fermentation time course, nitrogen  sources, carbon sources, phosphate sources, different salts and their  concentrations for getting optimal yield of triacylglycerol hydrolase. Utilization of  olive oil for production of lipases is a conventional approach, which is quite costly  for commercial applications; therefore, palm oil was incorporated in cultivation  medium as an alternative cheap substrate for triacylglycerol hydrolase production.  Enzyme yield from Pseudomonas stutzeri SN-3 was optimized by  using 1 gm% palm oil and 4 gm% yeast extract as carbon and nitrogen sources  respectively in the presence of 2 gm% CaCl2 as enzyme stabilizer and 0.01%  KH2PO4 as bacterial growth promoter. The maximum enzyme production was  observed after 48 hours of fermentation with medium pH 7 at 37 ⁰C. Conclusively,  we had a novel Pseudomonas stutzeri SN-3 specie and a cost-effective and eco- friendly medium for commercial production of triacylglycerol hydrolase.