In vitro elicitation supports the enrichment of 2H4MB production in callus suspension cultures of D. hamiltonii Wight & Arn

Authors

  • UMASHANKAR KOPPADA Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India Author
  • PRADEEP MATAM Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India Author
  • GIRIDHAR PARVATAM Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India Author

DOI:

https://doi.org/10.25083/rbl/27.1/3302-3308

Keywords:

Biomass, Elicitor, Callus cell suspension cultures, Meta-topolin (N6-(meta-hydroxybenzyl) adenine), Sodium nitroprusside (SNP), Pectin

Abstract

Influence of elicitation on in vitro production of 2-Hydroxy-4-Methoxy Benzaldehyde (2H4MB), a structural isomer of vanillin from callus suspension  cultures of Decalepis hamiltonii was investigated. In vitro culture conditions were  optimized to induce callus, suspension culture and biomass followed by metabolite  production. Suspension cultures were established using leaf generated  friable callus. Maximum content of 2H4MB production 0.079 ± 0.01 mg  100g-1 DW and biomass 197.5 ± 1.5 gL-1 were observed by 4th week of culturing.  Elicitation was induced to suspension cultures by using m-topolin (mT), sodium nitroprusside (SNP), and pectin individually at different concentrations. m-topolin  (15 μM), pectin (15 μM) and SNP (10 μM) supported 0.31 mg100g-1 DW, 0.27mg100g-1 DW and 0.21 mg100g-1 of 2H4MB production respectively by 4th  week. This data infers that the elicitation improves 2H4MB content in callus  suspension cultures of D. hamiltonii. 

RBL271-15

Downloads

Published

2024-05-23

Issue

Vol. 27 No. 1 (2022): Romanian Biotechnological Letters

Section

Articles