In vitro elicitation supports the enrichment of 2H4MB production in callus suspension cultures of D. hamiltonii Wight & Arn

Autori

  • UMASHANKAR KOPPADA Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India Autor
  • PRADEEP MATAM Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India Autor
  • GIRIDHAR PARVATAM Plant Cell Biotechnology Department, Council of Scientific and Industrial Research – Central Food Technological Research Institute, Mysore – 570 020, Karnataka, India Autor

DOI:

https://doi.org/10.25083/rbl/27.1/3302-3308

Cuvinte cheie:

Biomass, Elicitor, Callus cell suspension cultures, Meta-topolin (N6-(meta-hydroxybenzyl) adenine), Sodium nitroprusside (SNP), Pectin

Rezumat

Influence of elicitation on in vitro production of 2-Hydroxy-4-Methoxy Benzaldehyde (2H4MB), a structural isomer of vanillin from callus suspension  cultures of Decalepis hamiltonii was investigated. In vitro culture conditions were  optimized to induce callus, suspension culture and biomass followed by metabolite  production. Suspension cultures were established using leaf generated  friable callus. Maximum content of 2H4MB production 0.079 ± 0.01 mg  100g-1 DW and biomass 197.5 ± 1.5 gL-1 were observed by 4th week of culturing.  Elicitation was induced to suspension cultures by using m-topolin (mT), sodium nitroprusside (SNP), and pectin individually at different concentrations. m-topolin  (15 μM), pectin (15 μM) and SNP (10 μM) supported 0.31 mg100g-1 DW, 0.27mg100g-1 DW and 0.21 mg100g-1 of 2H4MB production respectively by 4th  week. This data infers that the elicitation improves 2H4MB content in callus  suspension cultures of D. hamiltonii. 

RBL271-15

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Publicat

2024-05-23

Număr

Volum 27 Nr. 1 (2022): Romanian Biotechnological Letters

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