Research on the action of fenugreek extract on the growth of the pathogen Monilinia spp. in vitro

Abstract

Moniliosis caused by the species of the Monilinia genus attacks fruit tree orchards wherever seeds fruits tree and stone fruits tree species are cultivated, producing economically significant losses. Moniliosis is also present in Romanian orchards, requiring control of this disease because it can evolve both in the field and in storge conditions, too. The research aimed to test the antifungal in vitro activity of fenugreek extract against the pathogen Monilinia spp. Monilinia spp was isolated and successively replicated from fresh plant material, represented by appl fruits with specific sporodochia. The fenugreek extract (ska) was tested in concentrations of 3.3% (ska 3,3%) and 10% (ska 10%), comparing the results with the control variant. At the 10% fenugreek extract, the fungus did not grow in the first 3 days, registering a vegetative growth after 6 days of incubation. After 12 days of incubation with the
10% fenugreek extract, the diameter of the mycelium colonies was 6.3 mm compared to the control variant, where the value of the colony diameter was 56.3 mm. The effectiveness of the fenugreek extract at a concentration of 10%, as a percentage of inhibition, was 88.80%.