Streptomyces lasiicapitis KSA18 isolated from Saharan soil in Algeria effective against most bacteria and fungi

Authors

  • MOKHTAR BENREGUIEG Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria Author
  • DJALLAL EDDINE HOUARI ADLI Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria Author
  • TAHA AHMED BENABBOU Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria Author
  • KADDOUR ZIANI Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria Author
  • MOSTAPHA BRAHMI Laboratory of biotoxicology, pharmacognosy and biological recovery of plants, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria Author
  • AYSE OZDEMIR Department of Chemistry, Istanbul Technical University, Istanbul, Turkey Author
  • KERIMAN GUNAYDIN Department of biology, Istanbul university, Turkey Author
  • JOACHIM WINK Microbial Strain Collection, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig D-38124, Germany Author

DOI:

https://doi.org/10.25083/rbl/27.5/3713.3721

Keywords:

Saharian soil, Actinobacteria, Algeria, antimicrobial activity, Streptomyces lasiicapitis, GC-MS

Abstract

Saharan soil still represents a formidable source of microorganisms that produce bioactive substances. Twentythree Actinobacteria strains were isolated from sand samples collected from saharian field in Algeria. Isolates were evaluated for antimicrobial activity. After primary screening, 79% of the isolates showed antimicrobial activity. KSA18 strains were selected for their intense activity. Bioactive metabolite was extracted with ethyl acetate and tested against pathogens microorganisms using the disk diffusion method. The crude extract was partially purified by column chromatography and evaluated for antimicrobial activity. Fraction A1 showed good activity against Staphylococcus aureus (3.25 mg/mL) and Listeria monocytogenes (4 mg/mL). This strain has been identified as Streptomyces lasiicapitis by 16S rRNA sequencing. GC-MS analysis of crude extract showed the presence of about 17 different volatile compounds). Some of them could be directly responsible for antibacterial or antifungal activity. The most important compounds are Phenol, 2,4-Bis(1,1-Dimethylethyl), 3-Isobutylhexahydropyrrolo[1,2-A] Pyrazine-1,4-Dione, 2-methyloctacosane and dibutyl phthalate.
However, this study requires other approaches to identify the bioactive molecules present in the extract.

Author Biographies

  • MOKHTAR BENREGUIEG, Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

    Laboratory of biotoxicology, pharmacognosy and biological recovery of plants, University of
    Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

  • DJALLAL EDDINE HOUARI ADLI, Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

    Laboratory of biotoxicology, pharmacognosy and biological recovery of plants, University of
    Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

  • TAHA AHMED BENABBOU, Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

    Laboratory of biotoxicology, pharmacognosy and biological recovery of plants, University of
    Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

  • KADDOUR ZIANI, Department of Biology, Faculty of ciences, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

    Laboratory of biotoxicology, pharmacognosy and biological recovery of plants, University of
    Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

  • MOSTAPHA BRAHMI, Laboratory of biotoxicology, pharmacognosy and biological recovery of plants, University of Saida-Dr. Moulay Tahar, 20000 Saida, Algeria

    Department of Biology, Faculty of Science and Technology, Ahmed ZABANA University of Relizane

RBL275-5

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Published

2024-06-13

Issue

Vol. 27 No. 5 (2022): Romanian Biotechnological Letters

Section

Articles