In vitro propagation of Lobelia chinensis Lour. under different LED lights

Abstract

The current study was performed to verify the effect of light quality especially of light-emitting diodes (LEDs) on the in vitro regeneration of Lobelia chinensis. Nodal explants were cultured on MS medium supplemented with 30 g L-1 sucrose and treated with red (R), blue (B), white (W) combination red/blue (1:1; RB), and red/blue/green (1:1:1; RGB) LED sources. After 4 weeks of culture regeneration capabilities, growth characteristics, and bioactive compounds were evaluated. RB treatments were found suitable for shoot regeneration, shoot growth, and root regeneration from shoots. Photosynthetic pigments, total phenolics, flavonoid content, and DPPH, ABTS, and FRAP antioxidant activities were highest with plants regenerated under RB LED. HPLC analysis of plants revealed the highest accumulation of catechin and myricetin in the plants regenerated under RB light sources.