Diversity, pathogenicity and biocontrol efficacy of Pseudomonas syringae isolated from plants in northern Jordan

Abstract

Diversity, pathogenicity, and biocontrol efficacy of Pseudomonas syringae strains isolated from a wide range of habitats in Jordan were investigated. Thirty-five  infected plant samples (vegetable leaves and woody plant twig) were randomly  collected. King's B agar medium was implemented and 124 colonies of P. syringae  candidates were selected. Phenotypic, biochemical and pathogenicity tests were  further carried out to confirm the identity of P. syringae isolates. The frequency of  fluorescent isolates varied between 11- 50 %. Even though all isolates were shown to induce chlorosis of tobacco leaves, only two were able to macerate potato slices  causing their rotting. This may be due to variation in secretion of cellulase and  pectinase enzymes involve in pathogenicity as clearly discussed in this study.  Interestingly, 55% of isolates were found to clearly inhibit growth of E. coli.  Exceptional inhibition was noticed in P. syringae isolated from tomato and a  possible effect of syringomycin was suggested. Therefore, P. syringae obtained in this study was recommended to have a pivotal role in the biological control  system. Results obtained from the experiment of ice nucleation activity revealed  that this phenotypic feature dominates woody plants.